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Tubing on the nano-liquid chromatography (nano-LC) process, used for quite small flow capacities The inner diameter (ID) of the HPLC column is a vital parameter.[44] It could possibly impact the detection reaction when lessened a result of the lessened lateral diffusion of the solute band. It may also influence the separation selectivity, when move
, for instance, demonstrates retention occasions for 4 weak acids in two mobile phases with just about identical values for (P^ prime ). Even though the buy of elution is the same for each mobile phases, Each individual solute’s retention time is afflicted in different ways by the choice of natural and organic solvent.Acid–foundation chemistry
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